Identification of Klebsiella granulomatis strain K22-14 16S rRNA gene and its primer design

Klebsiella granulomatis is gram-negative bacteria of the genus Klebsiella which causes sexually transmitted disease (STD) Donovanosis and urinary tract infection in older persons. In the present work, in silico approach for primer designing has been implemented; to gather more information about the bacterium. Primer plays an important role to initiate the process of Polymerase Chain Reaction (PCR), which amplifies DNA sequences. Proper primer designing is one of the most important parameter for successful DNA sequencing. Plethora of bioinformatics programs for PCR primer design reflects the central role of PCR experiments in modern molecular biology and in the –omics era. The work summarizes the flowchart approach to design primer for Klebsiella Granulomatis strain, to study its characteristics and generate specific primer sequences using bioinformatics tool. Result reveals a dire need to develop strategies through wet lab experiments to obtain effective drug target for curation of this deadly disease Donovanosis.